Recombinant human C-C Motif Chemokine 3 (MIP-1-alpha) protein (MI-290)


Macrophage Inflammatory Protein 1-Alpha (MIP-1-alpha) is part of the CC subfamily of the chemokine superfamily of chemoattractant cytokines. MIP-1-alpha affects the inflammatory response of blood monocytes, tissue macrophages, and T cells. MIP-1-alpha also enhances the formation of granulocyte-macrophage colonies stimulated by GM-CSF or M-CSF. Human MIP-1-alpha is composed of 69 amino acid residues with not possible N-linked glycosylation sites, and several possible O-linked sites. Human MIP-1-alpha is 60% homologous with human MIP-1-beta, 75% homologous with murine MIP-1-alpha, and has been shown to be active on murine cells. Human recombinant MIP-1-alpha is produced in genetically engineered yeast, and purified by sequential chromatography. This recombinant protein has a size of 8 kDa.
Alternative names C C motif chemokine 3, CCL 3, CCL3, CCL3_HUMAN, Chemokine C C motif ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, G0S19 1 protein, Heparin binding chemotaxis protein, L2G25B, LD78 alpha, LD78-alpha(4-69), LD78alpha, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP 1A, MIP-1-alpha, MIP-1-alpha(4-69), MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, small inducible cytokine A3 (homologous to mouse Mip-1a), Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
Type Recombinant.
Source Yeast


Product name Recombinant human C-C Motif Chemokine 3 (MIP-1-alpha) protein (MI-290)
Catalog number MI-290-3
Brand family Austral Biologicals
Product type Proteins and Peptides

Biological Information

Purity Over 95% (SDS gel electrophoresis, with low level of endotoxin).
Concentration 1 mg/ml
Purification method Purified by sequential chromatography


SwissProt ID Link P10147
Gene Aliases CCL3, G0S19-1, MIP1A, SCYA3
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV)
Subcellular Location Secreted
Post-Translational Modification N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells
Subunit Structure Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69)
Sequence Similarities Belongs to the intercrine beta (chemokine CC) family

Poduct Presentation

Form Liquid solution
Preservative No preservatives added
Storage buffer DI water

Storage and Shipping Information

Storage Store at -80 ºC

References and Citations

Citations 1. Nathan, C.F. (1987)J. Clin. Invest. 79:319.
2. Wolpe S.D. et al. (1989) Proc. Natl. Acad. Sci. (USA) 86:612.
3. Tekamp-Olson. P. et al. (1990) J. Exp. Med. 172:911.
4. Dunlop, et al (1992) Blood 79: 2221.
5. Suzuki, K. et al. (1989) J. Exp. Med. 169:1895