Recombinant human Macrophage Colony-Stimulating Factor 1 (M-CSF) protein (GF-390)

$200.00


Human M-CSF is produced in genetically engineered E. coli cells, and purified by sequential chromatography, refolding and HPLC. This polypeptide is a disulfide-linked dimeric protein. This recombinant protein has a size of 55 kDa. 
Alternative names Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, M-CSF, Macrophage colony stimulating factor, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930, Processed macrophage colony-stimulating factor 1
Type Recombinant.
Source E. coli


Description

Product name Recombinant human Macrophage Colony-Stimulating Factor 1 (M-CSF) protein (GF-390)
Catalog number GF-390-3
Brand family Austral Biologicals
Product type Proteins and Peptides


Biological Information

Purity Over 92% (amino acid composition and SDS gel electrophoresis)
Concentration 1 mg/ml
Purification method Purified by sequential chromatography and reverse-phase HPLC


Target

SwissProt ID Link P09603
Function Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance
Subcellular Location Cell membrane; Single-pass type I membrane protein. Secreted › extracellular space
Involvement in Disease Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers.

Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection
Post-Translational Modification N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated
Subunit Structure Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R


Poduct Presentation

Form 0.1% Mannitol, 0.1M Citrate (pH 6.5)


Storage and Shipping Information

Storage Store at -20 ºC


References and Citations

Citations 1. Halenbeck, R. et al. (1989) Biotechnology 7: 710-714
2. Kawasaki, E.S. et al. (1985) Science 230: 291:296
3. Suzu, S. et al. (1989) Cancer Res. 49:5913-5917