Recombinant human Epidermal Growth Factor (EGF) protein (GF-010)

$80.00


Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human EGF1-52.
Alternative names Beta urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, URG, Urogastrone
Type Recombinant.
Source Yeast


Description

Product name Recombinant human Epidermal Growth Factor (EGF) protein (GF-010)
Catalog number GF-010-5
Brand family Austral Biologicals
Product type Proteins and Peptides


Biological Information

Purity Over 97% (N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis)
Purification method Purified by sequential chromatography and reverse-phase HPLC


Target

SwissProt ID Link P01133
Gene Aliases EGF
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro
Subcellular Location Membrane; Single-pass type I membrane protein
Involvement in Disease The disease is caused by mutations affecting the gene represented in this entry.

Disease description: A disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to moderate psychomotor retardation, and brisk tendon reflexes
Post-Translational Modification O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor)
Tissue Specificity Expressed in kidney, salivary gland, cerebrum and prostate.
Subunit Structure Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF2 (By similarity). Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD)
Sequence Similarities Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats


Poduct Presentation

Form Lyophilized powder.


Storage and Shipping Information

Storage Store at +4 ºC. Reconstitution is best in water or buffer near neutral pH


References and Citations

Citations 1. Cohen, S. (1962) J, Biol. Chem. 237:1555
2. Gregory, H. (1985) J.Cell Science Supp.. 3:11
3. George-Nascimento, C. et al. (1988) Biochemistry 27:797-802