Anti-Axl antibody (AA22733)

$287.00


Alternative names Tyrosine-protein kinase receptor UFO, EC 2.7.10.1, AXL oncogene, AXL, UFO, UFO_HUMAN, P30530, 558
Source 894
Species Reactivity Human, Mouse, Rat
Applications IP, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-Axl antibody (AA22733)
Catalog number AA22733
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Tyrosine-protein kinase receptor UFO antibody, EC 2.7.10.1 antibody, AXL oncogene antibody, AXL antibody, UFO antibody, UFO_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human AXL
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:1000 IP 1:20 - 1:50
Purification method Purified by affinity chromatography


Target

Target Name Axl
SwissProt ID Link P30530
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
Subcellular Location Cell membrane; Single-pass type I membrane protein.
Involvement in Disease AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thyroid tumorigenesis. Overexpression of AXL and its ligand was also detected in many other cancers such as myeloproliferative disorders, prostatic carcinoma cells, or breast cancer.
Post-Translational Modification Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion. Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
Tissue Specificity Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Subunit Structure Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TNS2. Part of a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.
Sequence Similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.