Anti-AXIN2 antibody (AA26684)

$187.00


Alternative names Axin-2, Axin-like protein, Axil, Axis inhibition protein 2, Conductin, AXIN2, AXIN2_HUMAN
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-AXIN2 antibody (AA26684)
Catalog number AA26684
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Axin-2 antibody, Axin-like protein antibody, Axil antibody, Axis inhibition protein 2 antibody, Conductin antibody, AXIN2 antibody, AXIN2_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human AXIN2
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Purification method Purified by affinity chromatography


Target

Target Name AXIN2
SwissProt ID Link Q9Y2T1
Function Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
Subcellular Location Cytoplasm.
Involvement in Disease Colorectal cancer: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine: Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. The disease is caused by mutations affecting the gene represented in this entry.
Post-Translational Modification Probably phosphorylated by GSK3B and dephosphorylated by PP2A. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
Tissue Specificity Expressed in brain and lymphoblast.
Subunit Structure Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Interacts with SMAD7 and RNF111. Interacts with ANKRD6.
Sequence Similarities Contains 1 DIX domain. Contains 1 RGS domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.