Anti-ATP5F1 antibody (AA29951)


Alternative names ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATPase subunit b, ATP5F1, AT5F1_HUMAN, P24539, 515
Source 256
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Product name Anti-ATP5F1 antibody (AA29951)
Catalog number AA29951
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names ATP synthase F(0) complex subunit B1, mitochondrial antibody, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1 antibody, ATP synthase subunit b antibody, ATPase subunit b antibody, ATP5F1 antibody, AT5F1_HUMAN antibody

Biological Information

Immunogen Recombinant protein of human ATP5F1
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:100
Purification method Purified by affinity chromatography


Target Name ATP5F1
SwissProt ID Link P24539
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Subcellular Location Mitochondrion. Mitochondrion inner membrane.
Subunit Structure F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. Component of an ATP synthase complex composed of ATP5F1, ATP5G1, ATP5E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5A1, ATP5B, ATP5D, ATP5C1, ATP5O, ATP5L, USMG5 and MP68.
Sequence Similarities Belongs to the eukaryotic ATPase B chain family.

Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3

Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.