Anti-ATP-Citrate Lyase antibody (AA22877)

$287.00


Alternative names ATP-citrate synthase, EC 2.3.3.8, ATP-citrate, pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY, ACLY_HUMAN
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, WB
Host Rabbit
Conjugated Unconjugated


Description

Product name Anti-ATP-Citrate Lyase antibody (AA22877)
Catalog number AA22877
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names ATP-citrate synthase antibody, EC 2.3.3.8 antibody, ATP-citrate antibody, pro-S-)-lyase antibody, ACL antibody, Citrate cleavage enzyme antibody, ACLY antibody, ACLY_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ACLY
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:1000 IF 1:20 - 1:50
Purification method Purified by affinity chromatography


Target

Target Name ATP-Citrate Lyase
SwissProt ID Link P53396
Function ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Subcellular Location Cytoplasm.
Post-Translational Modification ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).
Subunit Structure Homotetramer.
Sequence Similarities In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family. In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family. Contains 1 ATP-grasp domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.