Anti-ATF6 antibody (AA22268)

$187.00


Alternative names Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, ATF6, ATF6A_HUMAN, P18850, 22926
Source 670
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ATF6 antibody (AA22268)
Catalog number AA22268
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Cyclic AMP-dependent transcription factor ATF-6 alpha antibody, cAMP-dependent transcription factor ATF-6 alpha antibody, Activating transcription factor 6 alpha antibody, ATF6-alpha antibody, ATF6 antibody, ATF6A_HUMAN antibody


Biological Information

Immunogen Synthetic peptide of human ATF6
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Purification method Purified by affinity chromatography


Target

Target Name ATF6
SwissProt ID Link P18850
Function Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein.; Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.
Post-Translational Modification During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR). Phosphorylated in vitro by MAPK14/P38MAPK.
Tissue Specificity Ubiquitous.
Subunit Structure Homodimer and heterodimer with ATF6-beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). Interacts also with the transcription factors GTF2I, YY1 and SRF. Interacts (via lumenal domain) with THBS1. Interacts with THBS4 (via EGF-like 3; calcium-binding domain) which facilitates its processing, activation and nuclear translocation (PubMed:22682248). Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner (PubMed:17765680).
Sequence Similarities Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP (basic-leucine zipper) domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.