Anti-ASF1A antibody (AA37209)

$187.00


Alternative names Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, hAsf1, hAsf1a, CCG1-interacting factor A, CIA, hCIA, ASF1A, CGI-98, HSPC146, ASF1A_HUMAN, Q9Y294, 25842
Source 204
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ASF1A antibody (AA37209)
Catalog number AA37209
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Histone chaperone ASF1A antibody, Anti-silencing function protein 1 homolog A antibody, hAsf1 antibody, hAsf1a antibody, CCG1-interacting factor A antibody, CIA antibody, hCIA antibody, ASF1A antibody, CGI-98 antibody, HSPC146 antibody, ASF1A_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ASF1A
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Purification method Purified by affinity chromatography


Target

Target Name ASF1A
SwissProt ID Link Q9Y294
Function Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Subcellular Location Nucleus.
Post-Translational Modification Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.
Tissue Specificity Ubiquitously expressed.
Subunit Structure Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1B, IPO4 and histones H3.1 and H4. Interacts with CREBBP.
Sequence Similarities Belongs to the ASF1 family.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.