Anti-ARHGEF7 antibody (AA23567)

$187.00


Alternative names Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB, Nbla10314, ARHG7_HUMAN, Q14155, 8874
Source 803
Species Reactivity Human, Mouse, Rat
Applications IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ARHGEF7 antibody (AA23567)
Catalog number AA23567
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Rho guanine nucleotide exchange factor 7 antibody, Beta-Pix antibody, COOL-1 antibody, PAK-interacting exchange factor beta antibody, p85 antibody, ARHGEF7 antibody, COOL1 antibody, KIAA0142 antibody, P85SPR antibody, PAK3BP antibody, PIXB antibody, Nbla10314 antibody, ARHG7_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ARHGEF7
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200
Purification method Purified by affinity chromatography


Target

Target Name ARHGEF7
SwissProt ID Link Q14155
Function Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Subcellular Location Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection, lamellipodium. Detected at cell adhesions. A small proportion is detected at focal adhesions.
Post-Translational Modification Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1. Phosphorylated on Ser-694 by CaMK1; enhancement of GEF activity and downstream activation of RAC1.
Subunit Structure Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and TGFB1I1. Interacts with PTK2/FAK1 and RAC1. Interacts with ITCH and PARVB. Interacts with unphosphorylated PAK1. Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1. Interacts with BIN2. Isoform 1 and isoform 5 interact with SNX27.
Sequence Similarities Contains 1 CH (calponin-homology) domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.