Anti-ARHGAP10 antibody (AA29795)

$287.00


Alternative names Rho GTPase-activating protein 10, GTPase regulator associated with focal adhesion kinase 2, Graf-related protein 2, Rho-type GTPase-activating protein 10, ARHGAP10, GRAF2, RHG10_HUMAN
Species Reactivity Human, Mouse
Applications IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ARHGAP10 antibody (AA29795)
Catalog number AA29795
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Rho GTPase-activating protein 10 antibody, GTPase regulator associated with focal adhesion kinase 2 antibody, Graf-related protein 2 antibody, Rho-type GTPase-activating protein 10 antibody, ARHGAP10 antibody, GRAF2 antibody, RHG10_HUMAN antibody


Biological Information

Immunogen Synthetic peptide of human ARHGAP10
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:200-1:2000 IHC 1:50-1:200
Purification method Purified by affinity chromatography


Target

Target Name ARHGAP10
SwissProt ID Link A1A4S6
Function GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.
Subcellular Location Cytoplasm. Cytoplasm, perinuclear region. Cell membrane. Association to cell membrane is dependent on PH domain.
Post-Translational Modification Phosphorylated. Phosphorylated in vitro by constitutive active PKN3.
Tissue Specificity High levels of expression in heart and skeletal muscle.
Subunit Structure Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity.
Sequence Similarities Contains 1 BAR domain. Contains 1 PH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.