Anti-APTX antibody (AA34252)

$187.00


Alternative names Aprataxin, EC 3.-.-.-, Forkhead-associated domain histidine triad-like protein, FHA-HIT, APTX, AXA1, APTX_HUMAN
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-APTX antibody (AA34252)
Catalog number AA34252
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Aprataxin antibody, EC 3.-.-.- antibody, Forkhead-associated domain histidine triad-like protein antibody, FHA-HIT antibody, APTX antibody, AXA1 antibody, APTX_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human APTX
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Purification method Purified by affinity chromatography


Target

Target Name APTX
SwissProt ID Link Q7Z2E3
Function DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity.
Subcellular Location Nucleus, nucleoplasm. Nucleus, nucleolus. Upon genotoxic stress, colocalizes with XRCC1 at sites of DNA damage. Colocalizes with MDC1 at sites of DNA double-strand breaks. Interaction with NCL is required for nucleolar localization.; Isoform 12: Cytoplasm.
Involvement in Disease Ataxia-oculomotor apraxia syndrome: An autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. The disease is caused by mutations affecting the gene represented in this entry.
Tissue Specificity Widely expressed. In brain, it is expressed in the posterior cortex, cerebellum, hippocampus and olfactory bulb. Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2.
Subunit Structure Interacts with single-strand break repair proteins XRCC1, XRCC4, ADPRT and p53/TP53. Interacts with NCL. Interacts (via FHA-like domain) with MDC1 (phosphorylated).
Sequence Similarities Contains 1 C2H2-type zinc finger. Contains 1 FHA-like domain. Contains 1 HIT domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.