Anti-APPL1 antibody (AA32324)

$287.00


Alternative names DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, APPL1, APPL, DIP13A, KIAA1428, DP13A_HUMAN
Species Reactivity Human, Mouse, Rat
Applications IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-APPL1 antibody (AA32324)
Catalog number AA32324
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names DCC-interacting protein 13-alpha antibody, Dip13-alpha antibody, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 antibody, APPL1 antibody, APPL antibody, DIP13A antibody, KIAA1428 antibody, DP13A_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human APPL1
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Purification method Purified by affinity chromatography


Target

Target Name APPL1
SwissProt ID Link Q9UKG1
Function Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
Subcellular Location Early endosome membrane; Peripheral membrane protein. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Post-Translational Modification Phosophorylation at Ser-410 by PKA severely impairs binding to OCRL.
Tissue Specificity High levels in heart, ovary, pancreas and skeletal muscle.
Subunit Structure Binds RAB5A/Rab5 through an N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Interacts with OCRL and INPP5B.
Sequence Similarities Contains 1 PH domain. Contains 1 PID domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.