Anti-APLF antibody (AA33913)

$287.00


Alternative names Aprataxin and PNK-like factor, EC 4.2.99.18, Apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, XRCC1-interacting protein 1, APLF, C2orf13, PALF, XIP1, APLF_HUMAN, Q8IW19, 200558
Source 511
Species Reactivity Human
Applications WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-APLF antibody (AA33913)
Catalog number AA33913
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Aprataxin and PNK-like factor antibody, EC 4.2.99.18 antibody, Apurinic-apyrimidinic endonuclease APLF antibody, PNK and APTX-like FHA domain-containing protein antibody, XRCC1-interacting protein 1 antibody, APLF antibody, C2orf13 antibody, PALF antibody, XIP1 antibody, APLF_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human APLF
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:200-1:2000
Purification method Purified by affinity chromatography


Target

Target Name APLF
SwissProt ID Link Q8IW19
Function Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
Subcellular Location Nucleus. Cytoplasm, cytosol. Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.
Post-Translational Modification Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1.Phosphorylated in an ATM-dependent manner upon double-strand DNA break.
Subunit Structure Interacts with LIG4, PARP1, XRCC1, XRCC4 and XRCC5.
Sequence Similarities Belongs to the APLF family. Contains 1 FHA-like domain. Contains 2 PBZ-type zinc fingers.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.