Anti-Apaf-1 antibody (AA23030)

$287.00


Alternative names Apoptotic protease-activating factor 1, APAF-1, APAF1, KIAA0413, APAF_HUMAN
Species Reactivity Human, Mouse, Rat
Applications IP, IHC, WB
Host Rabbit
Conjugated Unconjugated


Description

Product name Anti-Apaf-1 antibody (AA23030)
Catalog number AA23030
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Apoptotic protease-activating factor 1 antibody, APAF-1 antibody, APAF1 antibody, KIAA0413 antibody, APAF_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human APAF1
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Purification method Purified by affinity chromatography


Target

Target Name Apaf-1
SwissProt ID Link O14727
Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Subcellular Location Cytoplasm.
Tissue Specificity Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Subunit Structure Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain).
Sequence Similarities Contains 1 CARD domain. Contains 1 NB-ARC domain. Contains 15 WD repeats.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.