Anti-ANLN antibody (AA37197)

$187.00


Alternative names Actin-binding protein anillin, ANLN, ANLN_HUMAN
Species Reactivity Human
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ANLN antibody (AA37197)
Catalog number AA37197
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Actin-binding protein anillin antibody, ANLN antibody, ANLN_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ANLN
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Purification method Purified by affinity chromatography


Target

Target Name ANLN
SwissProt ID Link Q9NQW6
Function Required for cytokinesis (PubMed:16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. May play a significant role in podocyte cell migration (PubMed:24676636).
Subcellular Location Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Involvement in Disease Focal segmental glomerulosclerosis 8: A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. The disease is caused by mutations affecting the gene represented in this entry.
Post-Translational Modification Phosphorylated during mitosis. Ubiquitinated, and this requires FZR1/CDH1.
Tissue Specificity Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Subunit Structure Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.
Sequence Similarities Contains 1 PH domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.