Anti-ALKBH8 antibody (AA38981)

$187.00


Alternative names Alkylated DNA repair protein alkB homolog 8, EC 1.14.11.-, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA, carboxymethyluridine(34)-5-O)-methyltransferase ABH8, EC 2.1.1.229, ALKBH8, ABH8, ALKB8_HUMAN, Q96BT7, 91801
Source 664
Species Reactivity Human
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ALKBH8 antibody (AA38981)
Catalog number AA38981
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Alkylated DNA repair protein alkB homolog 8 antibody, EC 1.14.11.- antibody, Probable alpha-ketoglutarate-dependent dioxygenase ABH8 antibody, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8 antibody, tRNA antibody, carboxymethyluridine(34)-5-O)-methyltransferase ABH8 antibody, EC 2.1.1.229 antibody, ALKBH8 antibody, ABH8 antibody, ALKB8_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ALKBH8
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Purification method Purified by affinity chromatography


Target

Target Name ALKBH8
SwissProt ID Link Q96BT7
Function Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).
Subcellular Location Cytoplasm. Nucleus. Predominantly cytoplasmic.
Tissue Specificity Widely expressed, with highest expression in spleen, followed by pancreas and lung.
Subunit Structure Interacts with TRMT112.
Sequence Similarities Belongs to the alkB family. Contains 1 Fe2OG dioxygenase domain. Contains 1 RRM (RNA recognition motif) domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.