Anti-ALIX antibody (AA26159)

$287.00


Alternative names Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, PDCD6IP, AIP1, ALIX, KIAA1375, PDC6I_HUMAN, Q8WUM4, 10015
Source 868
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IP, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ALIX antibody (AA26159)
Catalog number AA26159
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Programmed cell death 6-interacting protein antibody, PDCD6-interacting protein antibody, ALG-2-interacting protein 1 antibody, ALG-2-interacting protein X antibody, Hp95 antibody, PDCD6IP antibody, AIP1 antibody, ALIX antibody, KIAA1375 antibody, PDC6I_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ALIX
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:200 - 1:2000 IF 1:50 - 1:100 IP 1:20 - 1:50
Purification method Purified by affinity chromatography


Target

Target Name ALIX
SwissProt ID Link Q8WUM4
Function Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
Subcellular Location Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
Post-Translational Modification May be phosphorylated on tyrosine residues by activated PDGFRB.
Subunit Structure Interacts with SH3KBP1. Interacts with PDCD6 and TSG101 in a calcium-dependent manner. Interacts with and SGSM3. Self-associates. Interacts with CHMP4A; the interaction is direct. Interacts with CHMP4B; the interaction is direct. Interacts with CHMP4C; the interaction is direct. Interacts with HIV-1 p6. Interacts with EIAV p9; the interaction has been shown in vitro. Interacts with CEP55; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry. May interact with PDGFRB. Interacts with SH3GL1 and SH3GL2. Interacts with murine leukemia virus Gag polyprotein (via LYPX(n)L motif). Interacts with murine leukemia virus Gag polyprotein (via LYPX(n)L motif).
Sequence Similarities Contains 1 BRO1 domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.