Anti-AKAP5 antibody (AA37185)

$187.00


Alternative names A-kinase anchor protein 5, AKAP-5, A-kinase anchor protein 79 kDa, AKAP 79, H21, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein, AKAP5, AKAP79, AKAP5_HUMAN, P24588, 9495
Source 427
Species Reactivity Human
Applications IF/ICC, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-AKAP5 antibody (AA37185)
Catalog number AA37185
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names A-kinase anchor protein 5 antibody, AKAP-5 antibody, A-kinase anchor protein 79 kDa antibody, AKAP 79 antibody, H21 antibody, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein antibody, AKAP5 antibody, AKAP79 antibody, AKAP5_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human AKAP5
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Purification method Purified by affinity chromatography


Target

Target Name AKAP5
SwissProt ID Link P24588
Function May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
Subcellular Location Membrane; Lipid-anchor. Associates with lipid rafts.
Post-Translational Modification Palmitoylation at Cys-36 and Cys-129 plays a key role in targeting to lipid rafts.
Tissue Specificity Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.
Subunit Structure Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C. Interacts with ADCY8, and enhances its phosphorylation at lipid rafts.
Sequence Similarities Contains 1 AKAP domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.