Anti-AFF4 antibody (AA32429)

$287.00


Alternative names AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Protein AF-5q31, Major CDK9 elongation factor-associated protein, AFF4, AF5Q31, MCEF, HSPC092, AFF4_HUMAN, Q9UHB7, 27125
Source 1163
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-AFF4 antibody (AA32429)
Catalog number AA32429
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names AF4/FMR2 family member 4 antibody, ALL1-fused gene from chromosome 5q31 protein antibody, Protein AF-5q31 antibody, Major CDK9 elongation factor-associated protein antibody, AFF4 antibody, AF5Q31 antibody, MCEF antibody, HSPC092 antibody, AFF4_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human AFF4
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:200-1:2000
Purification method Purified by affinity chromatography


Target

Target Name AFF4
SwissProt ID Link Q9UHB7
Function Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Subcellular Location Nucleus. Associates to transcriptionally active chromatin but not at snRNA genes.
Involvement in Disease A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia . Insertion ins(5;11)(q31;q13q23) that forms a KMT2A/MLL1-AFF4 fusion protein.
Tissue Specificity Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.
Subunit Structure Interacts with ELL3; the interaction is direct. Interacts with ELL2; the interaction is direct and leads to stabilize ELL2 and prevent ELL2 ubiquitination and degradation. Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL.
Sequence Similarities Belongs to the AF4 family.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.