Anti-AFAP1 antibody (AA29207)

$287.00


Alternative names Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP, AFAP1_HUMAN
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-AFAP1 antibody (AA29207)
Catalog number AA29207
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Actin filament-associated protein 1 antibody, 110 kDa actin filament-associated protein antibody, AFAP-110 antibody, AFAP1 antibody, AFAP antibody, AFAP1_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human AFAP1
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:1000 - 1:2000
Purification method Purified by affinity chromatography


Target

Target Name AFAP1
SwissProt ID Link Q8N556
Function Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Subcellular Location Cytoplasm, cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
Post-Translational Modification Phosphorylated on tyrosine residues by SRC.
Tissue Specificity Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
Subunit Structure Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers. Interacts with activated SRC SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB and PRKCI.
Sequence Similarities Contains 2 PH domains.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.