Anti-ADRB2 antibody (AA24041)

$287.00


Alternative names Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor, ADRB2, ADRB2R, B2AR, ADRB2_HUMAN, P07550, 154
Source 413
Species Reactivity Human, Mouse, Rat
Applications IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ADRB2 antibody (AA24041)
Catalog number AA24041
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Beta-2 adrenergic receptor antibody, Beta-2 adrenoreceptor antibody, Beta-2 adrenoceptor antibody, ADRB2 antibody, ADRB2R antibody, B2AR antibody, ADRB2_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human ADRB2
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:1000 IHC 1:50 - 1:100
Purification method Purified by affinity chromatography


Target

Target Name ADRB2
SwissProt ID Link P07550
Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Subcellular Location Cell membrane; Multi-pass membrane protein. Colocalizes with VHL at the cell membrane.
Post-Translational Modification Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation. Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor. Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent. Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.
Subunit Structure Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33. Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation. Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with CNIH4 (PubMed:24405750).
Sequence Similarities Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.