Anti-active Caspase 3 antibody (AA25997)

$187.00


Alternative names Caspase-3, CASP-3, EC 3.4.22.56, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, CASP3, CPP32, CASP3_HUMAN, P42574, 836
Source 277
Species Reactivity Human, Mouse, Rat
Applications IF/ICC, IP, IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-active Caspase 3 antibody (AA25997)
Catalog number AA25997
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Caspase-3 antibody, CASP-3 antibody, EC 3.4.22.56 antibody, Apopain antibody, Cysteine protease CPP32 antibody, CPP-32 antibody, Protein Yama antibody, SREBP cleavage activity 1 antibody, SCA-1 antibody, CASP3 antibody, CPP32 antibody, CASP3_HUMAN antibody


Biological Information

Immunogen Recombinant protein of human CASP3
Concentration 1 mg/ml
Isotype IgG
Application Notes WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Purification method Purified by affinity chromatography


Target

Target Name active Caspase 3
SwissProt ID Link P42574
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Subcellular Location Cytoplasm.
Post-Translational Modification Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Subunit Structure Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.
Sequence Similarities Belongs to the peptidase C14A family.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.