Anti-ACIN1 antibody (AA27464)

$187.00


Alternative names Apoptotic chromatin condensation inducer in the nucleus, Acinus, ACIN1, KIAA0670, ACINU_HUMAN
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-ACIN1 antibody (AA27464)
Catalog number AA27464
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names Apoptotic chromatin condensation inducer in the nucleus antibody, Acinus antibody, ACIN1 antibody, KIAA0670 antibody, ACINU_HUMAN antibody


Biological Information

Concentration 1 mg/ml
Isotype IgG
Purification method Purified by affinity chromatography


Target

Target Name ACIN1
SwissProt ID Link Q9UKV3
Function Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.
Subcellular Location Nucleus. Nucleus speckle. Nucleus, nucleoplasm. Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.
Post-Translational Modification Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1. Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.
Tissue Specificity Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.
Subunit Structure Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.
Sequence Similarities Contains 1 SAP domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 or -80 C. Avoid multiple freeze/thaw cycles.