Anti-AAK1 antibody (AA27038)

$187.00


Alternative names AP2-associated protein kinase 1, EC 2.7.11.1, Adaptor-associated kinase 1, AAK1, KIAA1048, AAK1_HUMAN, Q2M2I8, 22848
Source 961
Species Reactivity Human, Mouse, Rat
Applications IHC, WB
Host Rabbit
Class Polyclonal
Conjugated Unconjugated


Description

Product name Anti-AAK1 antibody (AA27038)
Catalog number AA27038
Brand family Ango
Product type Primary Antibody
Antibody Alternative Names AP2-associated protein kinase 1 antibody, EC 2.7.11.1 antibody, Adaptor-associated kinase 1 antibody, AAK1 antibody, KIAA1048 antibody, AAK1_HUMAN antibody


Biological Information

Concentration 1 mg/ml
Isotype IgG
Purification method Purified by affinity chromatography


Target

Target Name AAK1
SwissProt ID Link Q2M2I8
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Subcellular Location Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.
Post-Translational Modification Autophosphorylated.
Tissue Specificity Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Subunit Structure Interacts with alpha-adaptin, AP-2, clathrin, NUMB and EPS15 isoform 2. Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1. Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form.
Sequence Similarities Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.


Poduct Presentation

Form Liquid
Preservative 0.02% (w/v) sodium azide
Storage buffer PBS with 50% glycerol, pH 7.3


Storage and Shipping Information

Storage Store at -20 C. Avoid multiple freeze/thaw cycles.