Recombinant human Acidic Fibroblast Growth Factor (FGF 1) protein (GF-040)

$75.00


Acidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues. Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography. This recombinant protein has a size of 15.5 kDa.
Alternative names Acidic fibroblast growth factor, aFGF, Beta endothelial cell growth factor, Beta-endothelial cell growth factor, ECGF, ECGF beta, ECGF-beta, ECGFA, ECGFB, Endothelial Cell Growth Factor alpha, Endothelial Cell Growth Factor beta, FGF 1, FGF alpha, Fgf1, FGF1_HUMAN, FGFA, Fibroblast growth factor 1, Fibroblast Growth Factor 1 Acidic, GLIO703, HBGF 1, HBGF-1, HBGF1, Heparin binding growth factor 1, Heparin binding growth factor 1 precursor, Heparin-binding growth factor 1
Type Recombinant.
Source Yeast


Description

Product name Recombinant human Acidic Fibroblast Growth Factor (FGF 1) protein (GF-040)
Catalog number GF-040-3
Brand family Austral Biologicals
Product type Proteins and Peptides


Biological Information

Purity Over 95% (HPLC analysis and SDS gel electrophoresis)
Concentration 0.5 mg/ml
Purification method Purified by affinity chromatography


Target

SwissProt ID Link P05230
Gene Aliases FGF1, FGFA
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 Can induce angiogenesis
Subcellular Location Secreted. Cytoplasm. Cytoplasm › cell cortex. Cytoplasm › cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu2+ ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59
Post-Translational Modification In the nucleus, phosphorylated by PKC/PRKCD
Tissue Specificity Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle
Subunit Structure Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu2+-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Interacts with LRRC59. Interacts with CSNKA, CSNKB and FIBP. While binding with LRRC59, CSNKA and FIBP seem mutually exclusive, CSNKB and FIBP may cooperatively interact with FGF1. Forms a ternary complex with FGFR1 and ITGAV:ITGB3 and induces the recruitment of PTPN11 to the complex
Sequence Similarities Belongs to the heparin-binding growth factors family


Poduct Presentation

Form 10 mM Tris-HCl, 1.5 M NaCl, pH 7.0. Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage


Storage and Shipping Information

Storage Store at -80 ºC. It is also pobetaible to keep this protein at low pH (2-5) for a few days


References and Citations

Citations 1. Gimenez-Gallego et al., Biochem. Biophys. Res. Comm.(1986) 138:611
2. Thornton et. al. (1983), Science 222, 623
3. Gospadorowicz, D. (1987) Methods Enzymol. 147:106